Antibody Engineering Service
Antibody development service
SEKBIO is a leading provider of IVD recombinant antibody development services for customers across the globe. Our team of experienced scientists utilizes cutting-edge technologies to generate high-quality antibodies that meet the specific needs of our clients. We offer a wide range of services, including antigen design, antibody production, purification, and characterization. Our recombinant antibodies have proven to be highly sensitive and specific, making them ideal for use in diagnostic applications. With a focus on quality and customer satisfaction, we are committed to providing customized solutions that exceed our clients' expectations.
Our service process begins with a detailed consultation with our clients to understand their specific needs and requirements. Our experienced team of scientists works closely with the client to develop a customized project plan that addresses all aspects of the project.
De novo antibody sequencing is the process of determining the amino acid sequence of an antibody without prior knowledge of its sequence. It involves identifying the variable regions of the antibody, which are crucial for its function, through techniques like mass spectrometry and bioinformatics analysis. This method is particularly useful for discovering new antibodies or validating existing sequences.
"Sekbio can provide De novo antibody sequencing services free of charge to you. Payment will only be required if the expressed antibody meets your requirements!"
Phase | Description |
Phase 1 | The DNA synthesis of VH and VL sequences |
Subclone VH and VL inserts into expression plasmids Heavy chain (HC) and Light chain(LC) In order to express the variable regions of the heavy chain (VH) and light chain (VL) of an antibody, the VH and VL inserts are subcloned into specific expression plasmids designed for heavy chain (HC) and light chain (LC) production. This process involves inserting the DNA sequences encoding the VH and VL regions into the appropriate expression plasmids, which are specialized circular pieces of DNA that serve as carriers for the genes of interest. The expression plasmids are designed to contain regulatory elements, such as promoters and terminators, which control the expression of the VH and VL sequences in the host cell. This allows for efficient production of the VH and VL proteins. | |
Expression and Validation of Antibodies at Small Scale in a Mammalian System Small-scale antibody expression in a mammalian system typically involves introducing the genes encoding the antibody's heavy and light chains into mammalian cells, such as CHO (Chinese Hamster Ovary) cells, using transfection methods. The transfected cells then produce the antibody, which can be harvested and purified for validation. Validation of the expressed antibodies often involves analyzing their binding specificity, affinity, and functionality through techniques such as ELISA (Enzyme-Linked Immunosorbent Assay), Western blotting, to ensure that they meet the desired criteria for their intended application. | |
Phase 2 | Mid-scale expression of recombinant protein by co-transfection in HEK or CHO cells It is achieved through a process called co-transfection. Co-transfection involves simultaneously introducing multiple plasmids containing the genes encoding the recombinant protein, along with any necessary co-factors or regulatory elements, into the host cells. This allows for the efficient production of the recombinant protein at a larger scale compared to small-scale expression |
Purification of antibodies by protein G and validation The purification of antibodies typically involves the use of protein G, which is a protein that specifically binds to the Fc region of immunoglobulins, including antibodies. Protein G can be utilized as an affinity chromatography resin to selectively capture and isolate antibodies from complex biological samples. | |
Phase 3 | Large scale (1L) expression of recombinant antibodies by transient transfection in HEK cells |
Purification of antibodies by protein G and validation |
SEKBIO also provide antibody discovery service using hybridoma antibody technology. Additionally, protein sequencing services are available from various Chinese companies, and outsourcing antibody sequencing can be done to obtain the antibody sequence.
Protein Expression Service
If you already possess the antibody sequence, we can assist you with its expression and purification through our services.
SEKBIO offers comprehensive services for recombinant protein expression and purification to meet your research and application needs.
Our team has extensive experience and expertise in providing high-quality and efficient services to our clients. Our services include one-stop solutions from gene optimization to expression and purification, aiming to maximize the yield and purity of your recombinant proteins.
SEKBIO's core technologies include the use of various host systems, such as Escherichia coli and mammalian cells, for recombinant protein expression. We also provide customized protein purification strategies, including affinity chromatography, gel filtration, ion exchange, and reverse-phase chromatography, to meet the specific requirements of different proteins.
The expression of proteins in Escherichia coli (E. coli) is currently the most widely used and cost-effective system for protein expression. E. coli has a well-characterized genetic background, a robust expression system, ease of operation, fast growth, low cost, high expression levels, and good stability, making it highly suitable for expressing proteins from various species. It offers significant advantages, particularly in the production of small-molecule proteins. However, one challenge of prokaryotic expression in E. coli is the potential formation of insoluble inclusion bodies. With over a decade of experience in protein expression, including expertise in inclusion body refolding, SEKBIO can provide comprehensive one-stop services, ranging from codon optimization to recombinant protein expression and purification, ensuring optimal results for our clients.
At SEKBIO, we are committed to providing excellent customer service and high-quality recombinant protein products. We understand the importance of time and quality in biological research and applications, and therefore we guarantee to deliver satisfactory results with the fastest speed and optimal quality.
By choosing SEKBIO, you will have access to professional recombinant protein development services to boost the success of your research.
If you would like to learn more about our services and customized solutions, please contact us.
